The ATGL lipase cooperates with ABHD5 to mobilize lipids for hepatitis C virus assembly
Fig 8
Subcellular localization and lipid droplet association of ATGL variants.
(a) Immunofluorescence analysis of HA-tagged ATGL localization respectively to the lipid droplets (BODIPY 493/503) and trans-Golgi (p230 marker) in Lunet N hCD81 cells. (b) Intensity profiles of the green and red channels along the dotted line depicted in the merge pictures of panel a. (c) Quantification of ATGL accumulation in the nucleus, the cytoplasm and at the lipid droplet periphery. The heatmap shows the fold enrichment of the protein signal in each compartment as compared to the mean protein signal in the whole cell. Average of 3 experiments with at least 10 pictures per experiment.