The ATGL lipase cooperates with ABHD5 to mobilize lipids for hepatitis C virus assembly
Fig 6
WT HCV also depends on ATGL-mediated lipid droplet.
Lunet N hCD81 cells were lentivirally transduced with an ABHD5-targeting or irrelevant shRNA and infected 72 hours later with HCV Jc1. We harvested 48 hours later the cell lysates and supernatants (n = 5). (a) Intracellular HCV RNA was quantified from the cell lysates by qRT-PCR and normalized to the GAPDH housekeeping gene. (b) Extracellular HCV RNA was quantified from the cell culture supernatants by qRT-PCR. (c) Released infectivity was assessed by transferring the supernatants onto Huh-7.5 cells, culturing the cells for 48 hours and immunostaining them for HCV NS5A. We quantified the infected foci automatically as described in the Methods section.