Bartonella effector protein C mediates actin stress fiber formation via recruitment of GEF-H1 to the plasma membrane
Fig 6
BepCBhe recruits eGFP-GEF-H1 to the plasma membrane via binding of the FIC-OB domain to eGFP-GEF-H1 and binding of the BID domain to the plasma membrane shown by immunocytochemistry.
(A-B) HeLa cells were co-transfected with an expression plasmid for eGFP-GEF-H1 and the indicated plasmids for either (A) no expression or (B) expression of either 3xFLAG-BepCBhe, 3xFLAG-BepCBhe(FIC-OB), or 3xFLAG-BepCBhe(OB-BID). After 24 h, cells were fixed and stained by immunofluorescence labeling for FLAG and microtubule before being analyzed by fluorescence microscopy (scale bar = 25 μm). The x-z sections presented correspond to orthogonal cuts at the white lines displayed in the microtubule channel.