Triclosan depletes the membrane potential in Pseudomonas aeruginosa biofilms inhibiting aminoglycoside induced adaptive resistance
Fig 5
Triclosan reduces the membrane potential surge induced by tobramycin at 2-hours.
24-hr old biofilms were treated with triclosan (100 μM), or tobramycin (500 μM), alone and in combination for 2-hrs. Cells were stained with DiOC2(3) to measure the membrane potential. Dead or permeabilized cells were excluded from membrane potential analysis by parallel staining with the dead cell indicator TO-PRO™-3 iodide. The results are percent averages plus the SD (n = 4). Percent values indicate the average relative abundance of events within each gate normalized to the total number of events analyzed, excluding dead cells, artifacts, aggregates and debris. A one-way ANOVA followed by Dunnett’s multiple comparison post-hoc test was used to determine statistical significance between each treatment and the untreated control, and Bonferroni’s post-hoc test was performed to compare tobramycin vs tobramycin and triclosan. *, p<0.05. NS, not significant.