Role for the flagellum attachment zone in Leishmania anterior cell tip morphogenesis
Fig 5
Flagellum-to-flagellum connections contained FAZ membrane domain proteins.
(A-E) Images of FAZ2 null mutant cells connected via their flagella expressing FAZ proteins tagged with mChFP (magenta) representing the different FAZ domains. The flagellum membrane protein SMP1 is tagged with eGFP (green) and the DNA is stained with Hoechst 33342 (cyan). The enlarged image shows the flagellum-to-flagellum connection in detail, with FAZ5 and FLA1BP clearly overlapping with the connection. Scale bar, 5 μm.