A conformation-based intra-molecular initiation factor identified in the flavivirus RNA-dependent RNA polymerase
Fig 6
The reactivity and stability of DENV2 NS5 EC9 were not apparently affected by both types of mutations.
A) The reaction flow chart for the reactivity test (1) and the stability test (2). (B) A comparison of the EC9 to EC10 conversion for the WT and two representative mutants at 5 or 300 μM CTP concentration. The fraction of the 10-mer intensity was shown in each lane. C-D) A comparison of the EC9 stability upon high-salt challenge for the above three NS5 constructs. The fraction of 10-mer intensity (determined based on gels in panel C) as a function of challenge time was plotted (D) to estimate the apparent EC inactivation rate constant (kina) for each construct.