Novel partiti-like viruses are conditional mutualistic symbionts in their normal lepidopteran host, African armyworm, but parasitic in a novel host, Fall armyworm
Fig 2
Distribution of the partiti-like viruses in S. exempta.
(a,b) Relative quantification of SEIV1 (a) and SEIV2 (b) using β-actin and GAPDH as reference genes at different stages (n = 4) (ANOVA: SEIV1: F = 10.111, d.f. = 8,27, P < 0.0001; SEIV2: F = 100.73, d.f. = 8,27, P < 0.0001). (c,d) Relative quantification of SEIV1 (c) and SEIV2 (d) in different tissues of larvae (for hemolymph, n = 3; others, n = 6) (SEIV1: F = 26.827, d.f. = 3,17, P < 0.0001; SEIV2: F = 13.819, d.f. = 3,17, P < 0.0001). (e,f) Relative quantification of SEIV1 (e) and SEIV2 (f) in different tissues of female (n = 3) (SEIV1: F = 10.875, d.f. = 3,8, P = 0.0034; SEIV2: F = 6.681, d.f. = 3,8, P = 0.0143). (g,h) Relative quantification of SEIV1 (g) and SEIV2 (h) in different tissues of male (n = 3) (SEIV1: F = 11.114, d.f. = 3,8, P = 0.0032; SEIV2: F = 14.539, d.f. = 3,8, P = 0.0013). 1st = first instar-stage larvae, 2nd = second instar-stage larvae, 3rd = third instar-stage larvae, 4th = fourth instar-stage larvae, 5th = fifth instar-stage larvae, 6th = sixth instar-stage larvae, p = pupae, F = Female, M = Male, Hem = Hemolymph, Fa = Fat body, Gu = Gut, Cu = Cuticle, Le = leg, He = Head, Th = Thorax, Ab = Abdomen. Means ± SD. Different letters indicate statistically significance differences.