An improved animal model for herpesvirus encephalitis in humans
Fig 2
In vitro growth properties and cell-to-cell spread of mutant viruses.
(A) RK13 cells were infected with PrV-Ka and the respective virus mutants at a MOI of 3, and harvested 24 h p.i. Plaque assays performed on RK13 cells were used to determine the titer of virus progeny. (B) Plaque diameters of the different virus mutants were assessed on RK13 cells. 20 plaques each per virus mutant were measured and compared to plaque diameter of PrV-Ka set as 100%. Average values and standard deviations of three independent experiments are shown. Significant differences of virus mutants compared to PrV-Ka are indicated by an asterisk (*).