The clue is in the lipid A: Rapid detection of colistin resistance
Fig 2
Schematic diagram of the sample preparation process for the adapted MALDIxin test performed on the MALDI Biotyper Sirius system (Bruker Daltonics).
The total sample processing time using this approach is less than 15 minutes, allowing for immediate assessment of whether bacteria are resistant to polymyxin antibiotics, independent of the mechanism of resistance. In the published feasibility study [18], bacterial colonies were grown overnight on solid medium; in future clinical applications, bacteria could be obtained directly from biological samples, such as urine or blood. Figure created with BioRender.