Glutamate dehydrogenase (Gdh2)-dependent alkalization is dispensable for escape from macrophages and virulence of Candida albicans
Fig 6
Phagosomal acidification following phagocytosis.
(A) Wildtype (PLC005) and gdh2-/- (CFG279) cells were labeled with pHrodo, and co-cultured with RAW macrophages in HBSS at MOI of 3:1 (C:M); images were obtained at 30 min intervals for 4 h. Representative images and quantification of phagosome intensities for both strains. (B) (upper panels) Time lapse images showing the pH changes encountered by a single fungal cell phagocytosed by a RAW macrophage. Time = 0 min is arbitrarily set when a single C. albicans (gdh2-/-; CFG279) cell is about to be phagocytized by the macrophage. Black arrow indicates the macrophage containing the fungal cell followed for quantification of fluorescence intensities (lower panel). The time points with maroon color correspond to the time images in the upper panels were taken. The asterisk (*) and bracket indicate a period of time with apparent rapid changes in fluorescence intensity, the consequence of phagosomes moving in and out of focus (S12 Vid).