Chitin and chitosan remodeling defines vegetative development and Trichoderma biocontrol
Fig 5
Single knockout mutants show severe morphological changes and aberrant chitin deposition in patches with reduced chitin levels.
(A) Chitin deposition and tip morphology in leading hyphae of the peripheral zone of each colony was visualized by CFW staining in epifluorescence microcopy using the inverted agar method for live-cell imaging [91]; scale bar = 20μm. (B) CFW staining highlights aberrant chitin deposition and septal development in selected deletion mutants; scale bar = 10μm. (C) Altered cell wall chitin deposition in chs and cda deletion strains compared to the WT was investigated by semiquantitative fluorescence microscopy using CFW and CR as stains. A defined area of the tip apex and subapex (shown in B) of leading hyphae was analyzed by densitometry using the ImageJ software platform (http://rsb.info.nih.gov/ij/). Mean +/- SEM from n = 25–60 hyphae are shown.