Parallel quorum-sensing system in Vibrio cholerae prevents signal interference inside the host
Fig 5
Effects of ethanolamine and its analogs on CqsR quorum-sensing response.
HapR-dependent bioluminescence profiles (lux/OD600) were measured in a Δ3 cqsR+ strain in the presence of 10 mM, 1mM, 0.1 mM A) ethanolamine, B) serinol, C) L-alaninol, D) D-alaninol. Blank indicates LB medium without ethanolamine added. HapR-dependent bioluminescence profiles (lux/OD600) were measured in E) Δ3 cqsRD198V strain and F) Δ3 cqsRD171V strain in the presence of 10 mM, 1mM, 0.1 mM concentrations ethanolamine respectively. Each figure shows a representative profile of each condition with two biological replicates. Each experiment was performed independently at least two times.