A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development
Fig 8
Phagocytic capabilities of hemocytes perfused from newly emerged WT and KO females.
Phagocytic capacity of hemocyte from WT (A) and KO (B) lines. Data shown represents the frequency distributions of fluorescent pHrodo E. coli bioparticles per individual granulocyte (phagocyte). Two biological replicates were performed in which 10 granulocytes per mosquito from at least three fields were counted. In each replicate 5 mosquitoes were analyzed. C. Effect of biologically active and mutant mJHBP protein injection on the phagocytic activity (mean % ±SEM) of granulocytes in WT and KO lines. Two biological replicates were performed in which granulocytes were counted as in panels A and B. In each replicate, granulocytes from 7–12 mosquitoes were evaluated. Significance levels (Mann-Whitney U test) are indicated with asterisks (**** p ≤ 0.0001, * ≤ 0.05).