A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development
Fig 7
mJHBP-deficient mosquitoes exhibit impaired hemocyte development (lower hemocyte counts) and increased granulocyte differentiation.
Relative abundance (Mean number ± SEM) of total hemocytes (A), prohemocytes (B) and granulocytes (C) per female in newly-emerged WT and KO lines. Relative proportion (%) of prohemocyte (D) and granulocyte (E) subpopulations in in newly emerged WT and KO lines. Relative abundance (Mean number ± SEM) of total hemocytes (F), as well as the proportions of prohemocytes (G), and granulocytes (H) perfused from newly emerged WT and KO adults reared to eclosion after injection of fourth instar larvae with buffer or biologically active mJHBP. Significance levels (Mann-Whitney U) are indicated with asterisks (**** p ≤ 0.0001, *** ≤ 0.001, ** ≤ 0.01, * ≤ 0.05). In panels A-E two biological replicates of 10 female mosquitoes each were counted. In panels F-H, two biological replicates of 4–7 mosquitoes each were performed.