A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development
Fig 4
Effect of mJHBP deficiency on the expression of markers of immune function in hemocytes, fat body and midgut tissues.
Relative expression levels for defensin A (A) and cecropin A (B) in hemocytes from E. coli-challenged WT and KO females measured at 24 and 48 h post-infection (n = 6, 3 biological replicates of 2 individuals). Relative expression levels for nitric oxide synthase (NOS) in fat body (C) and hemocytes (D) from E. coli- challenged females measured at 24 and 48 h post-infection (n = 6, 3 biological replicates of two individuals). Relative expression levels for defensin A (E), cecropin A (F), attacin B (G), and gambicin (H) in midguts from E. coli-challenged WT and KO females measured at 24 and 48 h post-infection (n = 6, 3 biological replicates of 2 individuals). Data were analyzed using the Mann-Whitney U test (** p ≤ 0.01).