A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development
Fig 2
Effect of mJHBP deficiency on developmental and reproductive aspects in Wild Type (WT) and mJHBP-/- (KO) lines.
A. Mean time to pupation (days) from egg hatching (day 0) to the onset of pupation for WT and KO mosquitoes (4 biological replicates of 20 larvae). B. Mean duration of the pupal stage (days) for WT and KO mosquitoes (4 biological replicates of 20 pupae). C. Mean wing lengths (±SEM) for WT (n = 30) and KO (3 biological replicates of 10 adults) female mosquitoes. D. Mean number of eggs (±SEM) laid by WT and KO females (3 biological replicates of 9–10 adults). E. Quantification of triglyceride levels in WT and KO in newly emerged adult females (6 h, 12 h, 24 h, 48 h, 72 h and 96 h post adult emergence) (2 biological replicates of 2 mosquitoes for each time point). F. Mean JH III levels (±SE) in WT and KO mosquito hemolymph quantified by mass spectrometry (5 biological replicates, of 5 mosquitoes). None of these characteristics differed significantly (p ≤0.05) between WT and KO lines (Mann-Whitney U test).