Ecotin, a microbial inhibitor of serine proteases, blocks multiple complement dependent and independent microbicidal activities of human serum
Fig 3
E. coli ecotin inhibits the activation of pro-FD to FD in NHS.
Its efficiency is compared to that of our already published in vitro evolved MASP-3 inhibitor, TFMI-3. 1 μM ecotin increases the half-life of fluorescently labeled pro-FD in NHS from 3 hours to 10 hours, while 1 μM TFMI-3 prolongs that to ~40 hours.