Modulation of flagellar rotation in surface-attached bacteria: A pathway for rapid surface-sensing after flagellar attachment
Fig 7
Increased flagellar load in ΔfleN bacteria increases cAMP in a FimV-dependent manner.
(A) ΔfleN bacteria assemble increased numbers of polar flagella. Wild-type or ΔfleN cells in which endogenous fliC was replaced with the fliC(T394C) allele were labelled with maleimide-AlexFluor488 and visualized by phase and fluorescence microscopy. (B) cAMP levels measured by EIA. Intracellular cAMP levels were measured by EIA. Bars show mean ± S.D. of triplicate samples from a representative experiment of 3–5 independent assays.