Modulation of flagellar rotation in surface-attached bacteria: A pathway for rapid surface-sensing after flagellar attachment
Fig 5
Deletion of both P. aeruginosa adenylate cyclases is required to suppress FlhF(R251G)-mediated inhibition of swimming.
PAK or isogenic mutants lacking one or both of the P. aeruginosa adenylate cyclases were transformed with a plasmid driving constitutive expression of FlhF(R251G) or the empty vector, pUCP. Overnight cultures grown in VBM plus carbenicillin (200 μg/mL) were adjusted to an OD600 of ~0.3 and spotted to 0.3% LB agar plates supplemented with 250 μg/mL carbenicillin. Bacteria were incubated at 30°C for 20h before swimming zones were measured. The wild-type, cyaA, cyaB and cyaAB strains are isogenic.