G protein α subunit suppresses sporangium formation through a serine/threonine protein kinase in Phytophthora sojae
Fig 5
PsGPA1 overexpression inhibits the nuclear localization of PsYPK1.
(A) Microscopic analyses of P. sojae transformants expressing PsYPK1-GFP or PsYPK1ΔN–GFP in the wild-type recipient stain P6497 (marked as PsYPK1-GFP or PsYPK1ΔN–GFP, respectively) or in a PsGPA1 silenced or overexpression strain (marked as PsGPA1-M27/PsYPK1-GFP and PsGPA1-FLAG/PsYPK1-GFP, respectively). DAPI (4', 6-diamidino-2-phenylindole) staining was performed by adding DAPI to the cultures 5 min prior to the microscopic analysis. DIC: differential interference contrast; Merge: overlay of DIC, GFP fluorescence and DAPI staining. Bar, 20 μm. (B) Relative fluorescence intensity along the red arrows in A. Green line: PsYPK1-GFP or PsYPK1ΔN–GFP, blue line: nucleus (stained with DAPI).