Co-opting the fermentation pathway for tombusvirus replication: Compartmentalization of cellular metabolic pathways for rapid ATP generation
Fig 4
Tombusvirus infection induces the expression of Pdc1 and Adh1 mRNAs in N. benthamiana.
(A) Top panels: semi-quantitative RT-PCR analysis of the NbPdc1 mRNA level at 1.5 dpi in N. benthamiana leaves infected with either TBSV or mock-infected. (B) Semi-quantitative RT-PCR analysis of the NbPdc1 mRNA level at 3 dpi in N. benthamiana leaves infected with either CIRV or mock-inoculated. The samples were taken 3 days after tombusvirus inoculation. Second panel: RT-PCR analysis of the tubulin mRNA level in the same plants. Each experiment was repeated three times. Bottom panels: Ethidium-bromide-stained agarose gels show the comparable amounts of RNA loading, as shown for the ribosomal RNA. (C) Top panel: semi-quantitative RT-PCR analysis of the NbPdc1 mRNA level at 3 days post agroinfiltration in N. benthamiana leaves agro-infiltrated to express TBSV p33, CIRV p36 or no-expression control. See further details in panels A-B. (D-F) Semi-quantitative RT-PCR analysis of the NbAdh1 mRNA level at 1.5 dpi in N. benthamiana leaves infected with either TBSV or mock-infected or CNV- or mock-infected at 3 dpi. See further details in panels A-B.