Hinge length contributes to the phagocytic activity of HIV-specific IgG1 and IgG3 antibodies
Fig 5
In vitro and in vivo stability tests.
A. Serum stability of 447-52D IgG1 and IgG3 in 3x and 5x forms (n = 2, mean and SD plotted) as defined by phagocytic activity of THP-1 cells against JRFL SOSIP antigen-conjugated beads. AU: arbitrary units. B. Pharmacokinetic assessment of infused WT VRC01 IgG1, 3x, and 5x variants (n = 2–3, mean and SD are plotted along with 1 phase decay curve fit). C. Biodistribution of WT IgG1, 0x, 3x, 5x hinge variants as defined by relative levels of human IgG as compared to mouse IgG detected in each tissue. No human IgG could be detected (ND) in the PBS-infused mouse. Bars indicate means.