Somatic hypermutation to counter a globally rare viral immunotype drove off-track antibodies in the CAP256-VRC26 HIV-1 V2-directed bNAb lineage
Fig 2
One mutation is required to introduce breadth into CAP256.04 while mutations in CDRH1, CDRH2 and CDRH3 are required for potency.
(A) Comparison of the CDR sequences, including the length and charge of the CDRH3 of CAP256.04 (orange) and CAP256.25 (brown). The blue boxes highlights key off-track mutations. (B) Neutralization data using mutants and chimeras (indicated schematically above the table) between CAP256.25 and CAP256.04, with neutralization potency shown by color, as indicated in the key. Neutralization data represents the arithmetic mean titers (IC50, μg/mL) from at least two experiments.