Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion
Fig 7
Meq inhibits the phosphorylation and nuclear translocation of IRF7.
(A) DF-1 cells were left untreated or transfected with the empty vector or Meq-Flag plasmid and treated with IFN stimulatory DNA (ISD) for 12 h. Cell lysates were left untreated or treated with calf intestine alkaline phosphatase (CIP) for 1 h, and western blotting was performed with the indicated antibodies. The protein levels of phosphorylated TBK1 (p-TBK1) and phosphorylated IRF7 (p-IRF7) were normalized to those of actin; the p-IRF7 protein is indicated by an asterisk (*). (B) CEFs were infected with MDV-WT or MDV-dMeq (MOI = 0.1) for 12 h before immunoblot analysis with the indicated antibodies. (C) DF-1 cells were transfected with the indicated plasmids for 36 h before coimmunoprecipitation and immunoblot analysis with the indicated antibodies. (D) DF-1 cells were transfected with an empty vector or Meq-Flag plasmid, and 24 h later, cells were either left untreated or transfected with ISD for 12 h before confocal microscopy. (E) DF-1 cells were transfected and treated with ISD as indicated and the cell lysates were separated into cytoplasmic and nuclear extracts. The IRF7 protein levels in the cytoplasm and nucleus were analyzed by western blotting. The data represent results from one of the triplicate experiments. **: p < 0.01, ***: p < 0.001.