Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion
Fig 6
Meq impairs the recruitment of TBK1 and IRF7 to STING adaptor.
(A) DF-1 cells were transfected with the indicated plasmids for 36 h before coimmunoprecipitation and immunoblot analysis with the indicated antibodies. (B) The STING-HA plasmid was cotransfected with STING-Flag with or without Meq-Myc into DF-1 cells. After 36 h of transfection, coimmunoprecipitation and immunoblot were performed with the indicated antibodies. (C) The CEFs were first mock infected or infected with wild-type MDV (MDV-WT) or Meq-deficient MDV (MDV-dMeq) and then transfected with ISD for another 12 h. The cells were lysed and subjected to immunoprecipitation assays with the indicated antibodies.