Latency reversal agents affect differently the latent reservoir present in distinct CD4+ T subpopulations
Fig 4
Proportion of cells expressing HIV-1 RNA and p24 after viral reactivation in different CD4+ T cell subpopulations.
A. Proportion of cells transcribing HIV-1 RNA after viral reactivation with single LRAs in the following populations: TNA, TSCM, TCM, TTM, TEM and TTD. Medians are shown. B. Proportion of HIV-1+ cells per million cells in each CD4+ T cell subpopulation with all tested drugs and their combinations. C. Proportion of patients showing synergistic, antagonistic or additive effects (Bliss independence model) after HIV reactivation with combinations of LRAs are shown for each CD4+ T cell subset. Percentage of patients responding to LRAs interactions are shown for each cell subset. D. Proportion of HIV-1 RNA+ TCM cells expressing the viral protein p24. Black asterisks denote statistical significance compared with the negative control (media) using a Wilcoxon test. Red asterisks denote statistical significance compared with the combination of Romidepsin plus Ingenol using a Friedman test followed by Dunn’s post hoc tests. Medians and min to max ranks are represented in panels B and D. *p<0.05, **p<0.01.