Latency reversal agents affect differently the latent reservoir present in distinct CD4+ T subpopulations
Fig 1
HIV reactivation kinetics in the latently infected cell line J-Lat.
J-Lat cells were incubated for 50h with medium (R10), single LRAs or the combination of different families of these compounds; Panobinostat (PNB, 30 nM), Romidepsin (RMD, 40 nM), Ingenol (ING, 100 nM), Bryostatin-1 (BRY-1, 10 nM) and JQ1 (1 uM). Percentage of GFP+ (in green) and Annexin V+ (in red) cells was monitored each hour using the IncuCyte ZOOM live cell imaging system (Essen Bioscience). Dashed lines show the effect at 22h for the single drugs Panobinostat, Romidepsin and JQ1 (in yellow) and for Ingenol and Bryostatin-1 (in blue). Dotted lines represent 22h.