Metal-independent variants of phosphoglycerate mutase promote resistance to nutritional immunity and retention of glycolysis during infection
Fig 6
A metal-independent phosphoglycerate mutase enables glycolysis when S. enterica serovar Typhimurium is manganese-starved.
(A & B) Wild type Salmonella and ΔgpmA were grown in rich medium in the presence of (A) increasing concentrations of CP or (B) 240 μg/ml of WT CP or the ΔS1 and ΔS2 CP mutants (t = 8). (C-F) Wild type Salmonella and ΔgpmA were grown in defined medium (DM) supplemented with (C) glucose (DM + glucose), (D) Casamino acids (DM + AA), (E) glycerol (DM + glycerol) or (F) sodium pyruvate (DM + pyruvate) as a carbon source in the presence of increasing concentrations of CP (t = 8). * = p≤0.05 relative to wild type by two-way ANOVA with Sidak’s multiple comparisons test. n≥3. Error bars indicate SEM. Also see S4 Fig.