Metal-independent variants of phosphoglycerate mutase promote resistance to nutritional immunity and retention of glycolysis during infection
Fig 4
Loss of GpmA sensitizes S. aureus to manganese limitation in the absence of manganese transporters.
The growth of (A) wild type, ΔgpmA, ΔmntC ΔmntH and ΔmntC ΔmntH ΔgpmA and (B) wild type, ΔgpmI, ΔmntC ΔmntH and ΔmntC ΔmntH ΔgpmI were assessed in rich medium supplemented with 1 μM MnCl2 and 1 μM ZnSO4 in the presence of increasing concentrations of CP (t = 8). For these assays, the bacteria were precultured overnight in TSB. * = p≤0.05 by two-way ANOVA with Tukey’s multiple comparisons test. n≥3. Error bars indicate SEM. Strains are normalized to growth of parental strains, i.e., ΔgpmA and ΔgpmI are normalized to wild type and ΔmntC ΔmntH ΔgpmA and ΔmntC ΔmntH ΔgpmI are normalized to ΔmntC ΔmntH. C) Wild type S. aureus, ΔgpmA, ΔmntC ΔmntH, ΔmntC ΔmntH ΔgpmA containing either pOS1 plgt (plgt) or pOS1 plgt: gpmA (plgt: gpmA) were grown in rich medium supplemented with 1 μM MnCl2 and 1 μM ZnSO4 in the presence of increasing concentrations of CP (t = 8). For these assays, the bacteria were precultured overnight in TSB. * = p≤0.05 by two-way ANOVA with Tukey’s multiple comparisons test. NS = not significant. n≥3. Error bars indicate SEM. Also see S3 Fig.