Metal-independent variants of phosphoglycerate mutase promote resistance to nutritional immunity and retention of glycolysis during infection
Fig 2
Expression of a metal-independent phosphoglycerate mutase promotes resistance to manganese starvation.
(A) Wild type S. aureus Newman, ΔgpmA and ΔgpmI and (B) wild type S. aureus Newman and ΔgpmA containing either pOS1 plgt (plgt) or pOS1 plgt:gpmA (plgt:gpmA) were grown in rich medium in the presence of increasing concentrations of CP (t = 7). (C) Wild type USA300 JE2, ΔgpmA and ΔgpmI and (D) wild type USA300 JE2 and ΔgpmA containing either pOS1 plgt (plgt) or pOS1 plgt:gpmA (plgt:gpmA) were grown in rich medium in the presence of increasing concentrations of CP (t = 7). (E) Growth of wild type S. aureus Newman, ΔgpmA and ΔgpmI in the presence of 240 μg/ml of wild type CP as well as the ΔS1 and ΔS2 site mutants (t = 7). (F) Growth of wild type S. aureus Newman, ΔgpmA and ΔgpmI derivatives in NRPMI in the presence and absence of 1 μM MnCl2 or 1 μM ZnSO4 (t = 8). * = p≤0.05 relative to wild type and ΔgpmI by two-way ANOVA with Tukey’s multiple comparisons test. n≥3. Error bars indicate SEM. Also see S1 Fig.