STING is required for host defense against neuropathological West Nile virus infection
Fig 5
STING is required to program the adaptive immune response during WNV infection.
(A) Luminex analysis of cytokines and chemokines from serum in mock (PBS) and WNV infected mice at D4 and D8 post infection. n = 3–8 per condition. Dark red indicates values outside of (above) set scale. (B) CD4+ and CD8+ T cell frequency and absolute number in D8 post infection spleens (top). Flow schematic (bottom left) and CD4/CD8 ratio (bottom right). n = 4–8 per condition. Unpaired students t-test; p = 0.05*; p = 0.005*; p = 0.0005***). (C) Characterization of D8 pi splenic CD8+ T cell sub-populations (CXCR3+, Ki67+, CD44+ and NS4b+). Left: frequency; Right: Absolute number. n = 4–8 per condition. Unpaired students t-test; p = 0.05*; p = 0.005*; p = 0.0005***). (D) Characterization of D8 pi splenic CD4+ T cell sub-populations (CXCR3+, Ki67+, CD44+, CD73+ and CTLA-4+). Left: frequency; Right: Absolute number. n = 4–8 per condition. Unpaired students t-test; p = 0.05*; p = 0.005*; p = 0.0005***). (E) Splenic FoxP3- and FoxP3+ CD4+ T cells. Top: frequency; Middle; absolute number; Bottom: gating scheme. n = 4–8 per condition. Unpaired students t-test; p = 0.05*; p = 0.005*; p = 0.0005***). (F-G) Analysis of FoxP3+ (F) and FoxP3- (G) subpopulations (CXCR3+, Ki67+, CD44+, CD73+ and CTLA-4+). Left: frequency; Right: Absolute number. n = 4–8 per condition. Unpaired students t-test; p = 0.05*; p = 0.005*; p = 0.0005***). B-F: WT, wild type; ST-/-, STING-/-.