The Orthotospovirus nonstructural protein NSs suppresses plant MYC-regulated jasmonate signaling leading to enhanced vector attraction and performance
Fig 5
MYC2 and its homologs in Arabidopsis are essential regulators of host immunity responses against WFT.
(A) GUS staining of AtMYC2p-GUS and AtTPS10p-GUS seedlings after 24 h of thrips feeding. An untreated line was used as a control. Arrows indicate thrips feeding sites. Bars = 2 mm. (B) Thrips preference (as percentage recaptured WFT out of 50 released) between the mutants and WT control in a two-choice assay. Three-week-old Arabidopsis plants cultured in MS medium were used for the thrips two-choice assay. Data are mean percentages + SE, n = 6. **P < 0.01, Wilcoxon matched pairs tests. (C) β-ocimene is less attractive to thrips than mock treatment in a two-choice assay. Data are mean percentages + SE, n = 6. **P < 0.01, Wilcoxon matched pairs tests. (D) Thrips preference (as percentage recaptured WFT out of 50 released) between the 35S:YFP-NSs transgenic Arabidopsis lines (NSs-1; NSs-2) and mock control in a two-choice assay. Three-week-old transgenic Arabidopsis plants cultured in MS medium were used. Data are mean percentages + SE, n = 6. **P < 0.01, Wilcoxon matched pairs tests.