The Orthotospovirus nonstructural protein NSs suppresses plant MYC-regulated jasmonate signaling leading to enhanced vector attraction and performance
Fig 2
TSWV infection increases attractiveness to the thrips vector in a terpene-dependent manner.
(A) Representative GC/MS ion chromatograms of the headspace volatile compounds of control (mock-thrips) and TSWV-infected peppers (TSWV-thrips) after thrips infestation for 6 h. The peaks of specific products are marked with arrows in the left panel. Relative abundance of terpenes emitted after thrips infestation are showed in the right panel. Values are means + SE, n = 4. **P < 0.01, Student’s t-test. (B) Terpenes emitted by N. benthamiana after TSWV infection (under MeJA treatment). Values are mean relative amounts (percentage of internal standard peak area) ± SE, n = 4. **P < 0.01, Student’s t-test. (C) Thrips preference (as percentage recaptured WFT out of 50 released) on the pure monoterpenes (linalool, α-pinene, β-pinene) and solvent control (n-hexane) in a two-choice assay. Data are mean percentages + SE, n = 6. *P < 0.05 **P < 0.01, Wilcoxon matched pairs tests.