Non-canonical NLRP3 inflammasome activation and IL-1β signaling are necessary to L. amazonensis control mediated by P2X7 receptor and leukotriene B4
Fig 6
Casp-11-/- mice were more susceptible infection and LTB4 and IL-1β restored resistance of P2X7 receptor-/- mice to L. amazonensis.
C57Bl/6 (A, B, C, D, E, F), P2X7-/- (C, D, E, F), Casp-1/11-/- (A, B), and Casp-11-/- (A, B) mice were infected with 106 promastigotes of L. amazonensis for 28 days and were treated with or without 5 ng of LTB4 (C, D) or 300 pg of IL-1β (E, F) twice weekly, for 3 weeks. Lesions was determined by thickening (A, C, E) and number of parasites by LDA as described (B, D, F). A, B correspond to mean ± SEM of a group of 9 animals; C, D correspond to mean ± SEM n = 8 mice in two independent experiments; E, F correspond to mean ± SEM of n = 5–4 animals (*P < 0.05).