Non-canonical NLRP3 inflammasome activation and IL-1β signaling are necessary to L. amazonensis control mediated by P2X7 receptor and leukotriene B4
Fig 5
L. amazonensis control via LTB4 was Casp-11 dependent.
Peritoneal macrophages from C57Bl/6 (panels A-C, G, and H) and Casp-11-/- (D-F) mice were infected with promastigotes of L. amazonensis at a ratio of 10:1 (Leishmania:macrophage). After 4 hours, the free parasites were washed and after 24 hours infected cells were pretreated with 2 μM of Z-YVAD-FMK (G) or Z-LEVD-FMK (H) for 30 min. Subsequently, cells were treated with LTB4 (100 nM). Twenty-four hours later, the infection index was determined. Standard values represent means ± SEM of 3 independent experiments performed in triplicate. Arrows correspond to vacuoles with L. amazonensis and asterisks represent empty vacuoles. (*P < 0.05) compared to the control group (without treatment).