Non-canonical NLRP3 inflammasome activation and IL-1β signaling are necessary to L. amazonensis control mediated by P2X7 receptor and leukotriene B4
Fig 3
L. amazonensis control via IL-1β was dependent on IL-1R signaling.
Peritoneal macrophages from C57Bl/6 (A-C), ASC-/-(D-F), and IL-1R-/- (G-I) were infected with L. amazonensis promastigotes at the ratio of 10:1 (Leishmania:macrophage). After 4 hours, the free parasites were washed and after 24 hours, infected cells were treated with 100 pg/ml of IL-1β. Twenty-four hours later, cells were stained and the infection index was obtained. Normalized values represent means ± SEM of 3–4 independent experiments performed in triplicate. Arrows correspond to vacuoles with L. amazonensis and asterisks represent empty vacuoles. (*P < 0.05) compared to the control group (without treatment).