Gasdermin-D and Caspase-7 are the key Caspase-1/8 substrates downstream of the NAIP5/NLRC4 inflammasome required for restriction of Legionella pneumophila
Fig 5
CASP7 is important for restriction of L. pneumophila replication in vivo in the absence of CASP1/11.
(A and B) C57BL/6, Casp1/11–/–, Casp7/1/11–/–, Casp7+/–/1–/–/11–/–, Casp7+/–/1+/–/11+/–and Nlrc4–/–mice were infected intranasally with 105 wild type L. pneumophila, lungs were harvested at the indicated time points and homogenates were plated for CFU determination. Each dot represents a single animal, and the horizontal lines represent the averages. *, P<0.05. Data are presented for one representative experiment of four (A) and two (B) experiments performed with similar results.