Gasdermin-D and Caspase-7 are the key Caspase-1/8 substrates downstream of the NAIP5/NLRC4 inflammasome required for restriction of Legionella pneumophila
Fig 4
CASP7 is important for restriction of L. pneumophila replication in vivo in the absence of CASP1/11.
Bone marrow-derived macrophages from C57BL/6, Casp7–/–, Casp1/11–/–, Casp7/1/11–/–,Casp8/1/11/Ripk3–/–and Nlrc4–/–mice were infected with wild type L. pneumophila (WT Lp, A, B) or flaA mutants (C, D). Macrophages were infected at an MOI of 0.015 and bacterial replication was assessed by measurement of luminescence (RLU) emitted by luciferase-expressing bacteria (A, C) or by estimating bacterial CFU (B, D). *, P<0.05: compared to C57BL/6. #, P<0.05: compared to Casp1/11–/–. Data are presented for one representative experiment of four (A, C) and three (B, D) experiments performed with similar results.