Gasdermin-D and Caspase-7 are the key Caspase-1/8 substrates downstream of the NAIP5/NLRC4 inflammasome required for restriction of Legionella pneumophila
Fig 2
CASP7 is activated downstream of CASP8 in the NAIP5/NLRC4 inflammasome.
Bone marrow-derived macrophages from Casp1/11–/–, Asc/Casp1/11–/–, Casp8/1/11/Ripk3–/–and Casp7/1/11–/–mice were left uninfected (NI, open bars) or infected with wild type L. pneumophila (WT Lp, grey bars) or flaA mutants (black bars) at an MOI of 10 for 8 hours. (A) Caspase-7 cleavage was measured by western blot using anti-Casp7 p18 and anti-α-actin antibody. (B and C) Caspase-8 cleavage was measured by western blot using anti-Casp8 p18 and anti-α-actin antibody (B) and Caspase-Glo 8 Assay kit (C). *, P<0.05. Data are presented for one representative experiment of four (A) and three (B) and two (C) experiments performed with similar results.