Peer pressure from a Proteus mirabilis self-recognition system controls participation in cooperative swarm motility
Fig 4
The transcriptional shift observed in cells experiencing Ids mismatch occurs during consolidation periods between swarming.
A. A time-course graph showing mean swarm fluorescence intensity over time for two conditions: Δids carrying a chromosomal BB2000_0531 fluorescence reporter (MJT02) co-swarmed with wildtype (wildtype + MJT02, cyan squares) or co-swarmed with the Δids strain (Δids + MJT02, gray circles). Three biological repeats were performed. Fluorescence was measured over equivalent areas for each experimental condition. Error bars are standard deviations; a.u. means arbitrary units. Asterisks show statistically significant differences (p < 0.01) calculated through individual Mann-Whitney tests for each timepoint. B. Representative microscopy images phase contrast overlaid with Venus fluorescence (100x magnification) of wildtype + MJT02 (top) and Δids + MJT02 (bottom) co-swarms. Images shown are of the first active swarm phase (left, 1 hour) and the first period of consolidation (right, 2.5 hours). Scale bars = 10 μm.