Histamine release from intestinal mast cells induced by staphylococcal enterotoxin A (SEA) evokes vomiting reflex in common marmoset
Fig 7
Pharmacological effects on SEA-induced emesis.
Common marmosets were injected intraperitoneally with 200 mg/kg of DSCG (n = 6), 10 mg/kg of DPH (n = 6), 400 μg/kg of cetirizine (n = 6), 40 μg/kg of granisetron (n = 6), 200 μg/kg of granisetron (n = 6), 1000 μg/kg of granisetron (n = 6) or PBS (n = 11) and then administered 250 μg/kg of SEA 30 min later. To inhibit 5-HT synthesis, common marmosets were injected with PCPA (3500 mg/kg) over 2 consecutive days (n = 6) before SEA administration as described above. To deplete 5-HT in peripheral neurons without norepinephrine defects, common marmosets were pretreated with desipramine (25 mg/kg) for 60 min, then injected with 5 mg/kg of 5,7-DHT, followed by SEA administration as described above (n = 6). (A) The emetic response (percentage of vomited monkeys) and (B) its frequency were assessed as described in materials and methods. Each bar represents the mean ± SD. The statistical analysis was performed using Fisher’s exact test and Dunnett’s test, *P<0.05, **P<0.01, ***P<0.001.