A fungal ABC transporter FgAtm1 regulates iron homeostasis via the transcription factor cascade FgAreA-HapX
Fig 2
FgAtm1 regulates the activity of cytosolic Fe-S proteins.
(A) Determination of FgLeu1, FgAco1 and FgFum1 activities in mitochondria and cytosol of the wild type, ΔFgAtm1 and ΔFgAtm1-C. The FgLeu1, FgAco1 and FgFum1 activity of the wild type were set as 1. Each strain was cultured in CM at 25°C for 36 hours before activity determination. Means and standard errors were calculated from three repeats. Significance was measured using unpaired t-test (n.s. not significant, **p < 0.01, ***p < 0.001). (B) The growth and microscopic observation of colony edge of the wild type and ΔFgAtm1 on MM with or without 10 mM leucine, 70 mM NH4Cl, 10 mM sodium glutamate or 10 mM uric acid sodium at 25°C for 3 day or 7 days. Bar = 100 μm. (C) The content of total glutathione in mitochondria and whole cell of the wild type, ΔFgAtm1 and ΔFgAtm1-C. Means and standard errors were calculated from three repeats. Significance was measured using unpaired t-test (n.s. not significant, *p < 0.05).