EhP3, a homolog of 14-3-3 family of protein participates in actin reorganization and phagocytosis in Entamoeba histolytica
Fig 2
Live imaging montage showing dynamics of GFP-EhP3 in motile trophozoites.
(A) The montage depicts a time series of selected frames of fluorescent images of motile trophozoites expressing GFP-EhP3. A number of pseudopods showing increased fluorescent intensity of GFP-EhP3 marked by white arrow head can be visualized in different directions. (Scale bar, 10 μm). (B) The graph represents time course of intensity of GFP-EhP3 at selected ROI in pseudopods vs. cytoplasm. Fluorescent intensity gradually increased at pseudopods in comparison with cytoplasm of the cells. (C) Western blot analysis of GFP-EhP3 in the parasite lysate. 50 μg of the lysate prepared from E. histolytica cells expressing GFP-EhP3 and GFP alone, grown in presence of 30 μg/ml G418 was loaded in each lane and probed with anti-GFP antibody. Anti-GFP antibody detected GFP fused 53 kDa protein (EhP3 + GFP) band in GFP-EhP3 expressing cells as compared to 26 kDa band of GFP alone in control cells. Anti-EhCaBP1 antibody was taken as equal loading control.