A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis
Fig 2
The morphology of Hpa haustoria is altered in A. thaliana SNUPO mutants. Boxplots represent the percentage of multilobed haustoria among total haustoria on A. thaliana wild-type (Col-0), the indicated mutants and transgenic homozygous T3 complementation lines (pollux co, pPOLLUX:POLLUX; sec13 co, pSEC13:SEC13; shrk1 co, pUbi:ShRK1; shrk2 co, pUbi:ShRK2; LjSYMRK, pUbi:Lotus japonicus SYMRK-mOrange) 5 dpi (a + b), or 4, 5 and 7 dpi with Hpa isolate Noco2 (d). For each genotype, at least ten independent stretches of hyphae per leaf have been analysed on at least 5 leaves. On average, 1100 haustoria have been analysed per genotype. (c) Representative pictures of intracellular haustoria are displayed. Bars = 25 μm. (e) Upper panel: Light microscopy of Technovit section of A. thaliana wild-type (Col-0) or the shrk1 x shrk2 double mutant 7 dpi with Hpa isolate Noco2. Bars, 10 μm. Middle panel: Confocal light scanning microscopy of leaves of A. thaliana wild-type (Col-0) or the shrk1 x shrk2 double mutant stained with aniline-blue 5 dpi with Hpa isolate Noco2. For every haustorium, the entry point can be identified by the formation of the usually bright callose neck. Bars, 25 μm. Lower panel: Confocal light scanning microscopy of leaves of A. thaliana wild-type (Col-0) expressing RPW8.2-YFP, which localises to the perihaustorial membrane 7 dpi with Hpa isolate Noco2. *, round haustoria; +, multilobed haustoria; arrows, hyphae. Bars, 10 μm. Black circles, data points outside 1.5 IQR of the upper/lower quartile; bold black line, median; box, Interquartile Range (IQR); whiskers, lowest/highest data point within 1.5 IQR of the lower/upper quartile. Stars indicate significant differences to Col-0 (Wilcoxon-Mann-Whitney test with Bonferroni-Holm correction; *, p < 0.05; **, p < 0.01; ***, p < 0.001). Each experiment was performed at least three times independently.