NK cells negatively regulate CD8 T cells via natural cytotoxicity receptor (NCR) 1 during LCMV infection
Fig 5
Severe disruption of splenic architecture in absence of NCR1 during chronic LCMV infection.
(A-C) 2x104 P14 T cells (Ly5.1+) were transferred into WT and NCR1gfp/gfp mice followed by LCMV WE infection. After 8 days, spleens were harvested and prepared for confocal microscopy and pictures were taken with a 10x objective. (A) Overview of the splenic sections. Region of interest (ROI), dashed line. Scale bar 80 μm (B-C) Ratio of MFI of volume of B cell zones (B220+) and the volume of MFI of T cells found within B cell zone was determined. Shown is the ratio of volume between B cell zone-localized (B) CD3+ cells/ (C) P14 T cells (Ly5.1+) and the volume of the B cell zone, respectively. Data shown are mean + SEM of 61–66 B cell zones derived from 4–5 mice pooled from 2 experiments. ns, not significant, * p<0.05 (unpaired two-tailed t test). (D-F) 2x103 P14 T cells (Ly5.1+) were transferred into WT and NCR1gfp/gfp mice followed by LCMV docile infection. After 8 days, spleens were harvested and prepared for confocal microscopy. (D) Overview of the splenic sections. Region of interest (ROI), dashed line. Scale bar 70 μm (E-F) Ratio of MFI of volume of B cell zones (B220+) and the volume of MFI of T cells found within B cell zone was determined. Shown is the ratio of volume between B cell zone-localized (E) CD3+ cells/ (F) P14 T cells (Ly5.1+) and the volume of the B cell zone, respectively. Data shown mean ±SEM of 54–97 B cell zones from 8–9 mice pooled from 3 experiments * p<0.05, **** p<0.0001 (unpaired two-tailed t-test).