Nlrp3 inflammasome activation and Gasdermin D-driven pyroptosis are immunopathogenic upon gastrointestinal norovirus infection
Fig 2
MNV induces secretion of mature IL-1β by activating the caspase-1- and ASC-dependent Nlrp3 inflammasome.
Bone-marrow-derived macrophages (BMDMs) from (A-C) WT mice; (D-F) WT and Caspase-11-/- mice; (G-I) WT, Caspase-1/11-/- and ASC-/- mice; or (J-L) WT and Nlrp3-/- mice were left untreated (unprimed) or were TLR2-primed after which they were mock infected (control) or infected with either UV-inactivated or live MNV, both at a MOI 5. Cell culture supernatants collected after indicated time periods (A) or after 24h (D, G, J) were analyzed for secreted IL-1β by ELISA; and (B, C, E, F, H, I, K, L) cell lysates prepared 24h post-infection was immunoblotted for IL-1β and caspase-1 maturation. Data shown in (A, D, G, J) are the means ± SD of triplicate wells from a representative experiment out of at least two independent experiments. Data shown in (B, C, E, F, H, I, K, L) are representative for at least two independent experiments.