Proximity-dependent proteomics of the Chlamydia trachomatis inclusion membrane reveals functional interactions with endoplasmic reticulum exit sites
Fig 5
ERES proteins Sec16A and Sec31A are recruited to the C. trachomatis inclusion.
(A) Immunofluorescence microscopy of cells showing cellular distribution of Sec16A (anti-Sec16A, first column, green in merge), Chlamydia IncA (anti-IncA, second column, magenta in merge), and DNA (DAPI, third column, blue in merge). (B) Distribution of the COPII outer coat protein Sec31A (anti-Sec31A, first column, green in merge), IncA, and nuclei. Single channel images are displayed in inverted grayscale. Merged panels display all three-color channels. Protein distribution in uninfected HeLa cells are shown in the top row. Deconvolved images from cells infected with C. trachomatis L2 at 24 hpi are shown as a summation of z-series images (merged planes) or a single xy plane. Enlargements (far right) represent the regions marked with white boxes. Scale bars = 16 μm.