A model of chronic, transmissible Otitis Media in mice
Fig 6
The study of B. pseudohinzii pathogenesis in the middle ear.
Figure schematically depicts the timeline in the pathogenesis of B. pseudohinzii induced otitis media in C57BL/6J mice. The bacterial numbers are derived from bacteria recovered from the middle ears, and pathology severity scores [44] are based on histological observations of experimentally infected animals [Histopathologic severity grades—0 (no significant histopathological alterations); 1 (minimal); 2 (mild); 3 (moderate); or 4 (severe)]. Letters (a-g) indicate approximate points where the critical stages in the development of chronic otitis media begin, and which are amenable to study using the B. pseudohinzii-mouse infection model. [a: Nasopharyngeal colonization, b: Ascent of the Eustachian tube, c: Colonization of middle ear, d: Induction of acute inflammatory stage, e: Inflammation and mucoidal effusion, f: Acute inflammatory stage, g: Chronic infection stage].