Full restoration of specific infectivity and strain properties from pure mammalian prion protein
Fig 6
Protein-only recPrPSc seeds require cofactor molecules to convert immunopurified BV PrPC to PrPSc.
Western blots probed with anti-PrP mAb 27/33. Immunopurified M109 BV PrPC substrate was supplemented with, from left to right, PrP0/0 BH, RNA, purified lipid cofactor, or PBS and 1% Triton X-100 buffer (-cofactor), as indicated. All of the reconstituted reactions were then seeded with BV M109 protein-only recPrPSc (top), or Mo protein-only recPrPSc (bottom), and subjected to three-round sPMCA.