Full restoration of specific infectivity and strain properties from pure mammalian prion protein
Fig 3
Histopathology of inoculated M109 bank voles.
Representative microscopic images of brain sections of M109 bank voles stained with hematoxylin and eosin (H&E) or subjected to immunohistochemistry (IHC) with anti-PrP mAb 27/33, as indicated. Rows from top to bottom: asymptomatic control bank vole sacrificed 410 days after inoculation with a 10−1 dilution of the original 6 μg/mL recombinant sPMCA input seed (Mo cofactor recPrPSc) serially diluted 1:10 eighteen times in recombinant sPMCA reaction buffer to demonstrate that there is no remaining infectivity from the input seed; terminally ill bank vole sacrificed 134 days after inoculation with a 10−1 dilution of BV M109 cofactor recPrPSc (final concentration = 0.6 μg/mL); terminally ill bank vole sacrificed 99 days after serial passage of BV M109 cofactor recPrPSc (10−1 dilution of 10% w/v BH); asymptomatic bank vole sacrificed 403 days after inoculation with 10−1 dilution BV M109 protein-only recPrPSc (final concentration = 0.6 μg/mL); and a terminally ill bank vole sacrificed 113 days after inoculation with [protein-only→BH PrPSc] (10−1 dilution of round three of the BH sPMCA reaction). The inoculum volume used was 30 μL. Scale bar = 100 μm.